Anais do Simpósio Latino Americano de Ciências de Alimentos
Anais do 14 SLACA - Simpósio Latino Americano de Ciência de Alimentos

Xylooligosaccharides (XOs) are important oligomers made up of xylose units, interconnected through β-1,4 bonds. XOs have received particular interest in recent years by the food and pharmaceutical industries due to their functional properties, associated with fighting diseases and maintaining health. Therefore, the objective of this work is to evaluate the in vitro digestibility, prebiotic, and antioxidant effects, as well as the production of short-chain fatty acids (SCFAs) from xylooligosaccharides obtained by enzymatic hydrolysis of the bamboo culm. The xylan extracted from the bamboo stalk was submitted to enzymatic hydrolysis, using the Shearzyme cocktail by Novozymes®, which contains endoxylanases at the concentration conditions of 7.5 mg protein/g xylan, 50 ºC, pH 5.0 for 24 hours. The extract obtained showed a high concentration of XOs, approximately 5g/L, composed of xylobiose (2.39 g/L), xylotriose (1.31 g/L), xylotetraose (0.567 g/L), xylopentose (0.223 g/L) L) and xylohexose (0.18 g/L). The in vitro digestibility tests indicated that the XOs obtained presented a high and desirable resistance to digestion, considering that 97.65% of the XOS leave the simulated intestinal phase. The xylooligosaccharides showed an expressive prebiotic effect, promoting the growth of Lactobacillus acidophilus and Bifidobacterium bifidus, fermenting the XOs and producing SCFAs, highlighting acetate (1.64 g/L), propionate (0.03 g/L), and butyrate (0.02 g/L). These acids play important roles, fighting inflammation, protecting brain functions, inhibiting the development of cancer cells and pathogens. The XOs showed a high total antioxidant capacity at a concentration of 2 g/L, with an optical density greater than 3.0, in addition to eliminating 63% of the DDPH free radical. Thus, the results showed that the XOs produced from bamboo stalks have great prebiotic potential and excellent antioxidant activities and can be used in applications related to food and pharmaceuticals.