This study aimed to evaluate the (poly)amines content and adulterations in honey samples produced in São Paulo (SP) and Santa Catarina (SC) (Brazil). Amines (HPLC), isotopic analysis (CF-IRMS), and chemical quality index (CQI) data were recorded and submitted to PCA. The highest levels of Spm were identified in SC38 and SC35 (31.10 and 27.15 µg/100g, respectively) samples and in SP1 and SP2 (22.83 and 34.48 µg/100g, respectively) ones. These samples also showed higher content of Hist (18.07 and 13.64 µg/100 g, respectively to SC38 and SC35; 180.53 and 159.81 µg/100 g, respectively to SP1 and SP2). SP8 (Bauru) contains higher levels of Spd (30.71 µg/100 g) and lower of Put (1.39 µg/100 g). Higher Put content was detected in SC 18 and SC19 (188.65 and 130.49 µg/100 g, respectively) honeys, from Vale do Rio Uruguai. SP55 (Ourinhos) and SP58 and SP56 (Avaré) presented the highest content of Cad and Tir. High levels of Put and Cad may indicate food spoilage. In addition, Put and Tir can potentiate the effects of Hist and Cad. Honeys from SP presented CQI between 0.51 - 32.34 and from SC, 1.19 to 25.09. The best CQI was detected in honey from Ourinhos (SP) and from Vale do Rio do Peixe and Planalto Central (SC). According to isotopic analyzes (111 samples, SC and SP), 8 SC honeys showed higher values of δ13C and protein, which can be considered outside the classification (AOAC 998.12 method). The results showed that edaphoclimatic and relief conditions provide diversity of botanical species, contributing to the production of honeys with peculiar characteristic of origin. The bioamines and isotopic analyses, in combination with chemometric analysis, constitutes an investigative approach relevant to the identification of patterns for the typification of polyfloral honeys and rapid detection of changes in food chemical composition, resulting from adulteration.