The effect of cellulose nanofiber (CNF) from soybean straw on the rheological properties of hydrogels forming solutions (HFS) based on gelatin was investigated. CNF extraction was performed by enzymatic hydrolysis of 3 g soybean straw, previously submitted at alkaline treatment with 150 mL of sodium acetate buffer with 280 µL of an enzyme cocktail Optimash™ VR (DuPont Inc, USA) at pH 4.0 and 50°C/42h, followed by mechanical treatment (15000 rpm/5min) and sonication (70% amplitude/3 min). HFSs were prepared with 10 g of A-type gelatin/100 g of HFS and 0, 2.5, 5.0 and 7.5 of CNF/100 g of gelatin. Rheological analyses were carried out in a rheometer using parallel plate geometry (60 mm) with a gap of 1.0 mm at 25 °C. Steady flow behavior analysis was performed from 0 to 200 s-1, and dynamic analyses were carried out by temperature sweep from 5 to 40 °C and 40 to 5 °C, and by frequency sweep from 0.01 to 10 Hz at 5 and 40 °C. The HFS presented a shear-thinning behavior. The Power law model was fitted to experimental data (R20.98). The increase of CNF concentration (0-7.5%) increased the consistency index (0.021±0.002 to 0.133±0.024 Pa.s) and decreased the flow behavior index (0.928±0.020 to 0.695±0.014). In contrast, transitions temperatures were did not influenced by the CNF concentration (Tsol-gel=24.3±0.3-23.8±0.1 and Tgel-sol=31.8±0.0-31.5±0.0 ℃). At 5℃, the storage modulus (G’) of the HFS was greater than loss modulus (G’’), meanwhile at 40 ℃, G’’>G’, as expected, varying slightly as a function of the frequency, characteristic of a weak gel behavior. At 5℃, the increase of CNF concentration (0-7.5%) raised G’ (10.2±0.0 to 14.7±1.7 MPa) and G’’ (0.12±0.00 to 0.28±0.02 MPa), at 1 Hz. The CNF presence influenced the flow behavior of HFS but not viscoelastic and thermal properties.