Muruci (Byrsonima crassifolia L.) belongs to the family Malpighiacea and is widely distributed in the Amazon region. The fruits native to this region have become subject of much research attracting the interest of various industrial segments due to their nutritional and functional potential. This study aimed to determine the quality parameters and the chromatographic profile of the oil extracted from this fruit using solid-liquid extraction. The analyses were carried out according to AOCS standard methods (1998). The results obtained showed an average yield of 21.7%, and based on its acidity and peroxide indexes, its quality parameters were within the limits established by Brazilian law, with averages of 1.25 mg KOH/g and 1.17 (mEqkg-1), respectively. The chromatographic profile of the oil showed a predominance of unsaturated fatty acids (62%), mainly Omega 6 and Omega 9 fatty acids, and a variety of fatty acids of considerable importance to the food industry. These data show the attributes of the oil extracted based on its quality and conservation standards, the nutritional profile, and oxidative stability.