The gut homeostasis is ensured by a balance of pro- and anti-inflammatory cytokines secreted in the colonic mucosa. An imbalance on local cytokines, especially with increased production of pro-inflammatory, is one of the factors that can contribute to inflammatory bowel disease development (IBD). The consumption of fruits and vegetables, sources of polyphenols and fibers, have been associated to reduced risk of IBD. The present study aimed to evaluate the effect of Passion Fruit Peel (PFF), which is high in bioactive compounds, in the cytokine expression in colonic tissue and serum after TNBS induced colitis. Male Wistar rats (21 days old) were divided into Control and PFF groups (n=12). They were fed with the control and experimental diet; the colitis was induced (10 mg of TNBS dissolved in 0.25 ml of 50% ethanol) in 6 animals (per group) when they completed 77 days old. After 7 days they were died through exsanguinations by cardiac puncture. The cytokines (IL-1β, IL-6 and TNF-) were assessed through Elisa assay in tissue colonic homogenates and serum. Colonic homogenate of the PFF Colitis group showed decreased IL-1β and TNF- expressions compared to Control Colitis group. IL-6 expressions on homogenate, as well as in serum were not changed in treated animals. These results could be related to the synergic effect of the dietary fibers and polyphenols, or by the individual action of these two components presents on the PFF. The regularly intake of PFF appears to decrease the level of pro-inflammatory cytokines in inflamed colonic tissue.