Quercetin (3,5,7,3’-4’-pentahidroxi flavone), is a phenolic compound common in plants and is considered a major flavonoid present in the human diet. Several therapeutic properties of quercetin have been studied in the last years. Epidemiological evidences have suggested that the diets with higher quercetin levels have positive effects in the prevention and treatment of different diseases. Given the importance of quercetin in human nutrition, the purpose of this study was identify and quantify the levels of quercetin in pulps of tropical fruits, by HPLC. It was used pineapple (Ananas comosus), acerola (Malpighia emarginata D.C.), monbim (Spondias mombin L.), cashew apple (Anacardium occidentalle L.), guava (Psidium guajava), soursop (Annona muricata L.), papaya (Carica papaya L.), mango (Mangifera indica L.), passion fruit (Passiflora edulis Sims.), Surinam cherry (Eugenia uniflora L.), sapodilla (Manikara zapota L.) and tamarind (Tamarindo indica L.) pulps. The detection and quantification of quercetin was performed from the production of hot extracts. Analysis were conducted in HPLC, performed at 40º C and 350nm. The soursop pulp did not present quercetin in their constitution. The monbim and acerola pulps presented high levels of quercetin (μg/g), followed by Surinam cherry, representing potential sources for the extraction and use of this substance. This study provides novel information about the quercetin content of tropical pulp fruits, which is essential for the understanding of their nutraceutical potential.