Berries are small fleshy fruits widely consumed in our diet and have attracted much attention due to their potential human health benefits. Although it is already well established that berries are sources of bioactive compounds such as polyphenols and anthocyanins, the studies focused mainly on berries from temperate climates, mainly in the temperate regions of Europe, Asia and North America. To this end, the aims of the present study was to determine the bioactive compounds in the blackberry (Rubus spp.), red raspberry (Rubus idaeus), strawberry (Fragaria x ananassa) and blueberry (Vaccinium corymbosum) produced in the subtropical areas of the states of Minas Gerais and São Paulo, Brazil. The analysis performed were: β-Carotene and lycopene; total phenolic content – adapted Folin–Ciocalteu method; total flavonoid content - aluminum chloride colorimetric assay; total monomeric anthocyanin content - pH differential method and vitamin C - colorimetric method with 2,4-dinitrophenylhydrazine. The blackberry stands out among the fruits evaluated by exhibiting the highest levels of phenols (850.52 mg GAEs/100g f.w.), flavonoids (81.03 mg CE/100g f.w.) and anthocyanins (58.61 mg of cyanidin 3-glucoside equivalent/ 100 g of f.w.). The red raspberry and strawberry showed the highest levels of ascorbic acid (92.17 and 90.13 mg/100g f.w, respectively). In relation to the carotenoids, the fruits analyzed here were low in β-carotene and lycopene (0.21-1.57 and 0.12-1.11 mg/100g, respectively). Compared with berries grown in temperate climates the greatest difference was found in relation to ascorbic acid, as all fruits analyzed showed levels well above those found in the literature.