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EFFECTS OF PROCESS PARAMETERS ON THE RECOVERY OF COMPOUNDS WITH ANTIOXIDANT PROPERTIES FROM BANANA BRACTS (MAÇÃ VARIETY)
Karen Linelle de Oliveira Santos
Universidade Estadual de Campinas
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Create a topicBanana inflorescence is an abundant and low-cost agricultural waste from banana crop and a potential source of functional ingredients, rich in compounds with bioactivities including antidiabetic and antioxidant properties. This work reported the influence of physical-chemical parameters on recovering of bioactive compounds from banana bracts using an experimental design. According to CCRD analysis, the estimated regression coefficients demonstrated positive and significant effects of the independent variables m:v ratio (%), agitation and temperature on the recovery of bioactive compounds, resulting in high antioxidant activities of the extracts evaluated by DPPH, ABTS, FRAP and ORAC assays. Thus, the most adequate conditions to recover antioxidant compounds from banana bract were: agitation of 100 rpm, 37.5ºC as incubation temperature and m:v ratio of 0.5%. The experimental values for the responses under the chosen conditions were: 36.83 mg of catechin equivalent per g of banana bract (CE/g) for condensed tannins and 46.09 mg galic acid per g (GAE/g) for the total phenolic compounds. For antioxidant properties, the following responses were obtained: 149.68 μmol TE/g from FRAP assay, 93.72 μmol TE/g for DPPH assay, 94.25 μmol TE/g for ABTS assay and 30.29 μmol TE/g for ORAC assay. The high coefficients of determination (R2) ranging from 0.80 to 0.94 indicated a good fit for the models. These results demonstrated that physical-chemical parameters exert important effects on the recovery of bioactive compounds from bracts and that need to be studied for an optimized extraction.
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