The composition of microalgae can contribute to nutritious and functional diets. Among the functional compounds, chlorophylls are in focus since positive effects on human health have been established, related to their bioaccessibility and subsequent bioavailability. In addition to essential nutrients, we hypothesized that microalgae could be used as sources of bioavailable chlorophylls. Thus, this study determined the bioaccessibility of chlorophylls from three different products: isolated chlorophyll extract (ICE), wet ultrasonicated biomass (WUB), and whole dried biomass (WDB) of Chlorella vulgaris and Arthrospira platensis. Also, the intestinal uptake of ICE was investigated. The samples were submitted to in vitro digestion model according to the INFOGEST protocol, Caco-2 cells determined the intestinal uptake and chlorophyll were determined by HPLC-PDA-MS/MS. After in vitro digestion, all tested products showed bioaccessible chlorophylls. In Chlorella vulgaris, the total bioaccessibility results were as follows: ICE (6.44%), WUB (1.67%), WDB (0.09%). While for Arthrospira platensis, the total bioaccessibility of chlorophylls was 9.23% for ICE, 43% WUB and 0.11% WDB. In summary, from a nutritional perspective, these three types of ingredients/products (ICE, WUB and WDB) influence the promotion of chlorophyll bioaccessibility. In this way, the data suggest that chlorophylls bioaccessibility from ICE is more significant than that in WDB and WUB. Thus, ICE was considered the most efficient condition and subjected to uptake by intestinal cells. Specifically, uptake chlorophyll from Chlorella vulgaris ICE (15.65%) is more significant than Arthrospira platensis (15.32%). Therefore, Chlorella vulgaris should be used in the formulations when chlorophyll and its derivatives are the target molecules.