The mango Mangifera indica L. are among the most fruits produced in Brazil, only the pulp, about 60% of the fruit, is consumed, the remainder consists of peel and seed, approximately 20% each. Its production is seasonal, the peak of its harvest is excess supply for fresh consumption, thus processing has diminished this problem, as well as applying for other purposes, as obtaining biomolecules. Due to the high content of lipids and proteins, this study evaluated the potential of obtaining lipolytic enzymes (glycerol ester hydrolase, EC 3.1.1.3) from mango. The presence of lipase was observed in three parts of mango, peel, pulp and seed, it was verified by analyzing the activity following by titrimetric method. The enzymes were characterized biochemically, performing in a wide pH range from acid (4-5) to neutral (7). This samples showed optimal temperature for the activity, between 20ºC and 50ºC. Activity was observed on PNP-butyrate. For the first time lipase activity was detected in these fruit, the ease extraction, added the abundance and low cost of matrices, high activity (reaching 168.9 U/g/min for peel) over a wide range of pH and temperature, counted the affinity for different types, mad this lipases a potential alternatives to commercial lipases.