The culture of fruit has been important position in the national agriculture in recent years. The peelings and seeds of some fruits are considered as waste because the composition of these parts in especial fatty acids is unknown. Such a study has not been reported previously, the main objective of this study was to evaluate the composition of omega 3 and 6 of fatty acids in peelings, seed and pulp of araça boi (Eugenia stipitata). The fruits were separated in different parts and the total lipids were extracted and transesterified to chromatographic analysis. The results in the peeling, seed and pulp showed fatty acids saturated (SFA), monounsaturated (MUFA) and polyunsaturated (PUFA). The SFA were bigger in the peeling (46.23%) and seed (44.01%) while the MUFA were bigger in the seed (36.68%) and pulp (47.69%). However, the PUFA were bigger in the peeling (30.50%) and pulp (36.60%) than seed (8.30%). Furthermore, the fatty acids were separated in omega 3 (n-3) and 6 (n-6). The pulp and seed showed higher values for omega 3 and 6 series. These values were about 4 and 28%, respectively for n-3 and n-6 series. Recent works suggested that the ratio PUFA/SFA and n-6/n-3 were bigger than 0.4 and less than 4.0, respectively. So only the pulps presented values bigger than 4.0 for ratio n-6/n-3 and the seed less than 0.4 for ratio PUFA/SFA. In conclusion, all the parts of araça boi showed constituents of the important nutrition value, such as essential fatty acids for human diet.