Anais do Simpósio Latino Americano de Ciências de Alimentos
Anais do 14 SLACA - Simpósio Latino Americano de Ciência de Alimentos

Carob is a food used to replace cocoa that has several polyphenol compounds, and many of these bioactive compounds are related to antioxidant actions. Thus, the aim of this study is to verify whether the aqueous extract carob (AEC) modulates the insulin signaling pathway in C.elegans (DAF-16/FOXO). The carob was purchased in powder form from the local market, and an aqueous extraction was carried out for analysis. In order to characterize the phenolic compounds, chromatographic tests were carried out by HPLC and analyzes of total phenolic compounds, DPPH, FRAP in vitro and in vivo analysis, using the nematode C.elegans. In acute exposure, success and longevity assays were performed, per se and using the stressor agent (paraquat - 0.5 mM), in addition to lipid peroxidation (TBARS), gene expression (GST and DAF-16) and enzyme activity analyzes catalase. Thus, it can be observed AEC that the indefinite gallic acid (1099,30 µg. L-1 ± 87,16), syringic acid (99,05 µg. L-1 ± 23,48), coumaric acid (16,95 µg. L-1 ± 0,22) and quercetin (48,77 µg. L-1 ± 2,63). The extract presented 516.96μg gallic acid equivalent (GAE). mL-1 in the quantification of total phenolic compounds, an EC50 of 2.58 mg. mL-1 in DPPH radical reduction and a reducing antioxidant power of Fe3+ to Fe2+ of 8.59 mg. mL-1 of ferrous sulfate per gram of extract. In C.elegans, AEC promotes increased longevity, increased protection against paraquat, decreased levels of lipid peroxidation, decreased expression of the glutathione S-transferase gene and increased catalase activity. In addition, the AEC enabled the migration of daf-16 gene expression to the nucleus, a factor related to increased longevity. We conclude that the AEC, in the practices tested in C.elegans, enables a modulation of the DAF-16 pathway, activating antioxidant actions, probably due to the action of its bioactive compounds, increasing life expectancy.