Probiotics are generally considered safe for a healthy population. However, some probiotic strains can take advantage of weak immunity in vulnerable groups and turn into opportunistic pathogens. For this reason, guidance is needed to assess the safety of probiotics. Thus, the aim of this work was to evaluate the association between the presence of virulence characteristics in lactic acid bacteria and the vegetable food matrix from which it was isolated. Safety tests were carried out on lactic acid bacteria isolated from different types of fruit (apple, banana, grape and orange). These tests assess whether the isolates produce substances that can harm the host's health or that can favor the resistance or invasion of pathogens. Namely, hemolytic activity, production of histamine, tyramine, cadaverine, gelatinase, DNase and mucinolytic activity. There was an association (p < 0.05) between hemolytic activity and fruits of the examined isolates. Grape isolates had a greater potential to have hemolytic activity when compared to isolates from other fruit sources, while apple isolates had the least potential to have hemolytic activity. Hemolytic activity is a common virulence attribute among pathogens, therefore, this test is a primary requirement for safety assessment of probiotics. There was also an association (p < 0.05) between isolates with positive tyramine production and fruit origin. Grape isolates had a greater potential to produce tyramine than isolates from other fruits, while banana isolates had the least potential to produce tyramine. This is a biogenic amine produced from a precursor amino acid, tyrosine. High levels of tyrosine in the body cause blood pressure to rise, which is especially dangerous for people who have hypertension. These results suggest that the food matrix from which the microorganism was isolated may favor the expression of virulence characteristics.