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DEVELOPMENT OF MULTILAYER PARTICLES TO CO-ENCAPSULATE BIOACTIVE COMPOUNDS

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Hydrogel particles are examples of encapsulating system, produced by ionotropic gelation that can be applied to protect sensitive compounds. The combination of this traditional technique with electrostatic deposition can improve the protection and retention of bioactive compounds, besides encapsulating more than one compound. In this context, this work aimed to develop a biopolymeric structure to co-encapsulate anthocyanin and hydrolyzed collagen, considering the structuring properties of this peptide. Multilayer hydrogels were prepared by dripping different gellan gum solutions (F1: 0.5%; F2: 1.0 % or F3: 1.5 % (w/w)) with anthocyanin (0.05% (w/w)) in hydrolyzed collagen (HC) solutions (2.5%; 5.0 %; and 7.5% (w/w)), at pH 2.5, during 10 min to form the first layer. The second layer was formed by the electrostatic deposition of gellan at 0.2% (w/w), followed by reticulation promoted by calcium chloride (150 mM/pH 2.5). Between each deposition step, the particles were washed with deionized water. The particles were studied regarding their protein adsorption, moisture, anthocyanin retention and mechanical properties. High encapsulation efficiency (EE) was obtained in all formulations (> 84%), though anthocyanin was lost during the washing steps and layer process. Particles presented high moisture contend (> 95.5%), with an expressive protein adsorption in dry base (> 42%). F1 retained the higher HC content (68%), presenting also high EE (84.25%) and showing a good resistance to rupture (156,8 KPa). The peptide concentration did not affect the properties of the beads (EE, protein adsorption and mechanical properties). Results pointed out that particles produced with 0.5 % of gellan (F1) adsorbed with 2.5% HC was the best formulation. This work indicated that it was possible to produce particles to vehiculate more than one compound, using gellan gum. In this case, besides acting as a bioactive, the HC also presented an important structuring function.