The mono- and sesquiterpenes are the most abundant aroma compounds in the nature and are currently used in the pharmaceutical, cosmetics and food industry. Among these compounds, limonene and α-pinene are considered promising substrates for the application in biotechnology, due to their abundance and low cost. However, the use of such monoterpenes in biotransformation processes is still technically challenging due to some toxic characteristics of these compounds when used as substrates in biological processes. Thus, this work aims the isolation and selection of potential microorganisms for the bioconversion of limonene and α-pinene into commercial interesting compounds. The microorganisms were pre-grew in Yeast Malt Medium and then incubated in phosphate buffer for 48h at 30°C and 150 rpm of speed agitation. Limonene or α-pinene (0.5% v/v) was added sequentially as the sole carbon source. Cellular growth was evaluated by absorbance (at 600 nm) and dry weight (105ºC). Among the 25 microorganisms tested, the LBA-JA-EN-C2 strain presented the best growth curves in the presence of limonene and α-pinene. Satisfactory growth was observed after 8h of the addition of mainly α-pinene, reaching 10 mg/L of biomass, followed by a decrease with the exhaustion of the substrate, showing that the microorganism used the monoterpene as carbon source for its development. However, the LBA-JA-EN-C2 strain responded better to the addition of α-pinene with 8% increase of its biomass during the process, while in the presence of limonene there was a reduction of 47,92% of the initial biomass. These preliminary results shows that this new isolated strain can be used in subsequently tests aiming to evaluate the products obtained from the bioconversion of α-pinene.