Brazil is the most competitive country in poultry, with excellence. The method of cooling poultry consists on immersion in pre-chiller and chiller, this stage represents a point of great waste of water and opportunity for sustainability. The objective of this study was to compare the microbial load in the disposal system, 56,000 L water a day, in the cooling and with reuse, in a refrigerator located in the West of Paraná, with a capacity of 200,000 birds a day. Fifteen pre-chiller and chiller carcasses were collected with sterile material, totaling 240, trials for mesophiles and Enterobacteria were carried out in the official laboratory, as recommended by SIF, by the method of Compendium 4a ed. 2001, expressed in log10 CFU/g-1, and submitted to analysis of variance (ANOVA) and Tukey's test at 5% probability. The temperature, chlorine concentration and water flow were also collected. The results for pre-chiller / chiller mesophiles in 2015 (4,00ᵃ and 3,29ᵃ) and enterobacteria (2,57ᵃ and 0,92ᵃ) in 2016 respectively (4,02ᵃ and 3,01ᵇ) and (2,00ᵇ and 0.30ᵇ), shows that the use of water did not affect the microbial load, reducing two logs, according to circular 012/07/DICAO/CGI/DIPOA, without significant difference. Chlorine in 2015 (0.33 ppm), 2016 (0.75 ppm) in the chiller, with a 2016 increase, due to the maintenance of water potability, as the water flow increases, keeping to the standard of the legislation. The temperature and flow of pre-chiller / chiller water in 2015 (6.6 and 0.5 ° C), (1.57 and 1.05 L / bird), 2016 respectively (5.5 and 0.4 ° C), (1.77 and 1.12 L / bird). It is concluded that maintaining the water reuse system, within the standards of potability and temperature, the quality of the product will not be affected and it provided a reduction of 580,000 L/water per day, contributing to sustainability.