Orange juice compounds (SL) present various biological properties, such as antioxidant, antihypertensive, blood cholesterol lowering activity, and according to current in vitro studies possess a selective action under the intestinal microbiota. The objective of this work was to investigate the impact of orange juice chronic consumption on the intestinal microbiota and to evaluate the lipid and glucose profile in healthy women. Ten eutrophic women were evaluated for a period of 120 days, divided into three periods: (1st): basal (30 days); (2nd) intake of 300 mL / day SL (60 days); (3rd) cleaning without SL (30 days). Samples of blood and feces were taken at baseline and every 30 days until the end of the experiment. At the end of the 2nd period (60 days with SL) total anaerobes and Lactobacillus ssp was increased (p <0.05); NH4+ was reduced and the short chain fatty acids (p <0.05) was increased of 2 and 3 times. Regarding intestinal health, the volunteers had a reduction in the frequency of dry stools and effort to evacuate (p <0.05). Biochemical parameters showed decreased glucose (-6.5%), insulin (-33%) and insulin resistance (-44%). There was also reduction of total cholesterol (-14%), LDL (-16%) and triglycerides (-30%). After stopping SL ingestion (end of 3rd period), the biochemical parameters returned to the initial values, showing the effect of the juice while consumed. Studies have reported that a diet rich in polyphenols favors the modulation of the intestinal microbiota and the improvement of the biochemical profile. According to the results obtained in the present work and correlate them with literature, it can be concluded that SL showed a positive modulating effect on the intestinal microbiota and on the glucose and lipid profile of women healthy.