ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY FROM ARAÇÁ ESSENTIAL OIL
The Myrtaceae family is constituted by approximately 1.000 species from 26 genus that include lots of fruits consumed by native Brazilian people due to their medicinal properties and importance as food source. Among these fruits, araçá is an important plant to be studied because of the pleasant flavor and high levels of bioactive compounds which have inverse association between feed standards that include these compounds and the risk of development of chronic diseases like cancer, diabetes, cardiac disorders, coronary diseases and Alzheimers. Furthermore extracts of these fruits are being investigated to be employed in food industries like natural additives. The araçá essential oil (AEO) was obtained by supercritical extraction under 50 ºC, 250 bar and 5 g/min of CO2 flow during 6 h of extraction with 20 min of static time. To evaluate antimicrobial activity a sensitivity test using diffusion discs was employed, was noted that AEO exhibited inhibitory effect against Staphylococcus aureus and Bacillus cereus strains but it was not effective against Escherichia coli and Salmonella strains. To evaluate the antioxidant activity first was conduced a liquid-liquid extraction of the AEO to separate polar from non-polar compounds that could interfere in the analysis and this activity was evaluated by photometric method of reduction of DPPH- radical and the kinetics of the reaction was monitored until steady state of 11 different concentrations of AEO in methanol (400.000 ppm, 300.000 ppm, 200.000 ppm, 100.000 ppm, 50.000 ppm, 10.000 ppm, 5.000 ppm, 1.000 ppm, 500 ppm, 100 ppm and 50 ppm). It was noted that AEO has no significant antioxidant activity because for the highest concentration tested, the percentage of remaining DPPH- at the steady state was 61.37%. Thus it can be concluded that AEO is not an effective antioxidant but it is a promising antimicrobial agent against two tested pathogenic bacteria.