Staphylococcus species are a frequent cause of foodborne diseases and hospital infections. They are readily acquire drug resistance, and multiple drug-resistant isolates are spreading in veterinary and human healthcare. Therefore, the object of study was to evaluate the prevalence of coagulase-positive staphylococci (CPS) and their drug resistance in artisanal minas cheese (AMC). The CPS enumeration was performed according to the ABNT NBR ISO 6888-1/2016 and antimicrobial susceptibility test was conducted using 14 antibiotics commonly used for humans or livestock by the Kirby Bauer disk diffusion method following CLSI guidelines. The antibiotics tested were Cefaclor (CFC), Cefoxitin (CFO), Cefuroxime (CRX), Cefotaxime (CTX), Ceftazidime (CAZ), Ceftriaxone (CRO), Cefepime (CPM), Ciprofloxacin (CIP), Clindamycin (CLI), Linezolid (LNZ), Oxacilin (OXA), Teicoplanin (TEC), Vancomycin (VAN), Sulfazotrim (SUT). CPS were detected in all samples analyzed, with a population between 2.7 and 5.5 log (CFU / g) and a mean of 4.3 log. The 88 Staphylococci were isolated from 11 AMC samples from 11 different producers (1 sample per producer), the highest occurrence of resistance was found for OXA (82,2%), CLI (78,4%), CRX (78,4), followed by CTX (65,9%), CAZ (64,8%), CFO (63,6%), CFC (61,4%), CPM (59,5%), CRO (54,5%), SUT (35,9%) and CIP (34,1%). Only 4 isolates show sensitivity to 14 antibiotics, whereas 14 CPS were resistant to 11 antibiotics. The results for resistance to oxacillin justify the non-use of it as drug of choice for treatment of infections caused by Staphylococcus. CPS showed greater sensitivity to ciprofloxacin (62.9%) and sulfazotrim (59.4%). The high incidence of antibiotic resistance in addition to pose a potential risk to public health, evidence the widespread use of these substances and the presence of multi-drug resistant CPS in the production chain of artisanal minas cheese.