Bee pollen has gained space in the human diet because of the health benefits attributed to its consumption. In Brazil one research reported the presence of vitamin B1 in levels that reach the Dietary Reference Intakes (DRI) per serving but only samples from Pariquera-Açu city, State of São Paulo (Southeast of Brazil). So, it is desirable to obtain studies in other regions of the country in order to characterize this micronutrient in bee pollen. It is recognized that the physicochemical constitution has a direct correlation with the plants visited by the bees. The deficiency of thiamine (vitamin B1) leads to beriberi, which manifests neurological and cardiovascular diseases in humans. Maybe this regional product can be used as dietary supplement and this study aims to evaluate the content of vitamin B1 in 11 samples of dryed bee pollen harvested on different apiaries at Rio Grande do Sul State, southern Brazil, between 2011 and 2012. The samples were analyzed by High Performance Liquid Chromatography (HPLC). Vitamin B1 content ranged from 0.5±0.1 to 1.8±0.1 mg / 100 g of dryed bee pollen. According to Brazilian legislation a food can be considered source or rich in a particular vitamin, if it provides an amount equal or above 15% or 30%, respectively, of the DRI in the specified portion of the product that for pollen is 25 g. The thiamin’s DRI consumption is 1.2 mg / day for adults. It can be concluded that six samples were considered source and two samples were rich in vitamin B1.