Anais do 15° SLACAN - Simpósio Latino Americano de Ciência de Alimentos e Nutrição

Coffee is one of the most consumed beverages and holds a relevant global economic value as a crucial commodity. Research has associated coffee consumption to a range of health benefits, like antioxidant properties and antiproliferative effects. Nevertheless, any factors exert a direct influence over the chemical composition and biological activity of coffee, such as the coffee cultivar and the brewing method. The aim of this study is to evaluate the total phenolic content (Folin-Ciocalteu method), antioxidant activity (ABTS+ and ORAC methods) of coffee beverages and influence of bioactive compounds in prostate cancer cells - DU145 (MTT assay). Robusta coffee beans (Coffea canephora), roasted lightly, were used to prepare coffee beverages at the same concentration (1:20) and produced using different methods (aeropress, V60, clever, espresso), according to specific parameters. Aeropress had the highest content of phenolic compounds and antioxidant activity by ABTS+ (205.20±1.76 mg GAE/mL and 490.80±11.57 μmol TE/mL), followed by espresso (192.47±4.21 mg GAE/mL and 190.40±16.55 μmol TE/mL), clever (190.98±6.35 mg GAE/mL and 162.90±19.34 μmol TE/mL) and V60 (169.96± 6.17 mg GAE/mL and 134.00±9.01 μmol TE/mL). In relation to ORAC assay, aeropress and espresso showed similar responses (91.97±9.97μmol TE/mL and 105.92±8.74 μmol TE/mL p>0.05) and higher reducing power compared to Clever and V60 (±16.53 and ±63.94 times greater, respectively). Cell viability decreased in a dose and time dependent. The most antiproliferative effect were observed since 1000 μg/mL after 48h of treatment, around 70-80% reduction in cell viability of DU145 cells, with no significant difference between the methods. In conclusion, the results suggest that the combination of infusion and filtration methods can improve the extraction of bioactive compounds, thus optimizing the obtaining of beverages with greater antioxidant and adjuvant potential in the context of prostate cancer treatment.