POLYPHENOL-RICH GRAPE RESIDUE EXTRACT IN THE PREVENTION OF TNBS-INDUCED ULCERATIVE COLITIS IN RATS

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Detalhes
  • Tipo de apresentação: Pôster
  • Eixo temático: Ciência de Alimentos e Nutrição (CN)
  • Palavras chaves: Inflammatory Bowel Disease; Antioxidants; plant extract;
  • 1 Universidade Estadual de Campinas
  • 2 Departamento de Alimentos e Nutrição / Faculdade de Engenharia de Alimentos / Universidade Estadual de Campinas

POLYPHENOL-RICH GRAPE RESIDUE EXTRACT IN THE PREVENTION OF TNBS-INDUCED ULCERATIVE COLITIS IN RATS

Ana Paula da Fonseca Machado

Universidade Estadual de Campinas

Resumo

Ulcerative colitis (UC) is a chronic inflammatory bowel disease due to ulceration of the inner lining of the colon and/or rectum. Several medications have been used to treat UC; however, most of them have shown side effects and high costs. To solve this problem, alternative medicine has been employed, which includes the use of bioactive compounds derived from vegetable matrices. In this case, the objective of this study was to evaluate the anti-inflammatory and antioxidant properties of polyphenol-rich grape residue extract in the health improvement of induced UC in rats. The UC model was induced by rectal administration of trinitrobenzene sulfonic acid (TNBS). Treatment with a polyphenol-rich aqueous extract (10 mg total polyphenols/kg animal weight/day) began 2 weeks before colitis induction. After induction, experimental animals from the treatment groups continued to be treated with the extract, and control animals were treated with mesalazine (100 mg/kg animal weight/day) or water for 7 consecutive days. The anti-inflammatory effects of the extract were assessed by body weight, colonic weight and length, and disease activity index (DAI) score. The colonic levels of reduced glutathione (GSH), thiobarbituric acid reactive substances (TBARS), interleukin (IL)-1β, and tumor necrosis factor-α (TNF-α) were also determined to assess the effect of the extract. The treatment with grape residue extract slightly improved body weight loss, colon weight, and length, and moderately decreased DAI. In addition, IL-1β levels in colonic tissue were also decreased by the administration of the extract, while GSH content has been increasing. TBARS and TNF-α levels in the colon did not vary significantly between experimental groups. The findings of this study suggest that polyphenol-rich grape residue extract, at a higher dosage than that used, could be a candidate to decrease the inflammatory and oxidant profile of UC induced in rats.

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