BIOCHEMICAL AND TOXICOLOGICAL CHARACTERIZATION OF WASTES FROM Annona muricata (GRAVIOLA) AND OPTIMIZATION OF BIOETHANOL PRODUCTION

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Detalhes
  • Tipo de apresentação: Pôster
  • Eixo temático: Bioquímica e Biotecnologia de Alimentos (BB)
  • Palavras chaves: fruit residues; fermentation; response surfaces;
  • 1 Instituto de Química / Universidade Federal de Goiás
  • 2 Universidade Federal de Goiás
  • 3 Departamento de Bioquímica e Biologia Molecular / Universidade Federal de Goiás

BIOCHEMICAL AND TOXICOLOGICAL CHARACTERIZATION OF WASTES FROM Annona muricata (GRAVIOLA) AND OPTIMIZATION OF BIOETHANOL PRODUCTION

Armando Garcia Rodriguez

Departamento de Bioquímica e Biologia Molecular / Universidade Federal de Goiás

Resumo

Several fruits from Brazilian Central Plain are consumed both fresh, and also used for industrial derivatives manufacturing, such as ice-creams, jellies and liqueurs, generating a large amount of wastes, such as peels and seeds. Although rich in nutrients and thus potentially useful for biotechnological purposes, these wastes are extensively discarded without any other destination. In this work, the nutrient content, toxicity and the fermentative potential of peels and seeds from Annona muricata (graviola) were studied. Carbohydrate, protein and phosphate content were determined by spectrophotometric methods (Dubois, Bradford and Jacintho, respectively), showing the highest concentration of carbohydrates and proteins in peels (80 +/- 11.36 mg/g and 1.37 +/- 0.12 mg/g), while the highest concentration of phosphates were found in the seeds (0.11 +/- 0.006 mg/g). Toxicity was evaluated with Artemia salina bioassay, showing the presence of toxic acetogenin-like compounds in the seeds, but not in the peels. Optimal fermentation conditions were determined using an experimental design of two factors (peel/seed concentration and fermentation time) with three levels each, running each factor-level combination in 100 mL Erlenmeyer flasks, using yeast strain S-05 and determining three responses in each experiment: cell growth (X), carbohydrate consumption (S) and ethanol production (P). Obtained response surfaces showed the highest yield and growth values with 5% of peels and 9% of seeds after 48 hours of fermentation. These responses were slightly increased when fermentation process was runned in the presence of 0.1 % alpha-amylase, reaching ethanol yield values of 8 g/100 mL (peels) and 7.8 g/100 mL (seeds), suggesting that the presence of acetogenins in the seeds does not cause any interference on anaerobic fermentation process, since they act on mitochondrial electron transport, depending on oxygen, and so confirming the suitability of these wastes for bioethanol production.

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Autor

Armando Garcia Rodriguez

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