Anais do Simpósio Latino Americano de Ciências de Alimentos
Anais do 14 SLACA - Simpósio Latino Americano de Ciência de Alimentos

High amounts of dietary triglycerides may induce hypercholesterolemia, a major risk factor for cardiovascular disease. Triglycerides are absorbed as fatty acids and monoacylglycerols after pancreatic lipase action in the intestinal lumen. This study aimed to investigate the inhibitory activity of digested guarana powder aqueous extract (GPAE) on pancreatic lipase in vitro. The GPAE was subjected to simulated oral, gastric and intestinal in vitro digestion. The antioxidant capacity was evaluated by the Oxygen Radical Absorbance Capacity (ORAC). The pancreatic lipase inhibitory activity was determined using 4-methylumbelliferyl oleate (4-MUO) as a substrate. In a 96-well microplate, 25µL of samples were mixed with 50µL of 0.1mM 4-MUO solution in Tris-HCl buffer (13mM, 150mM NaCl, 1.3mM CaCl2, pH 8.0). The mixture was incubated with porcine pancreatic lipase type II solution (50 U/ml) in Tris-HCl buffer (30 min/37°C). The reaction was stopped by 100µL of 0.1M sodium citrate (pH 4.2). The amount of 4-methylumbelliferone released was measured in a fluorometrical microplate reader (SpectraMax M5 - Molecular Devices Inc, USA) at excitation of 355 nm and emission of 460 nm and the IC50 was calculated. Orlistat was used as a positive control (1.2 - 0.075 µg/mL). The antioxidant activity of the undigested GPAE was lower than that digested, with the final ORAC values of 2512 (±399) and 4321 (±778) µmol/Eq Trolox/g, respectively, demonstrating higher antioxidant activity after interaction with enzymes and digestive fluids. The pancreatic lipase inhibitory activity from GPAE proved to be dose dependent, with an IC50 value of 1033 µg/mL. In parallel, the IC50 value of Orlistat was 0.14 µg/mL. In conclusion, GPAE demonstrated an inhibitory activity of the pancreatic lipase that may contribute to reduce lipid absorption and presented great antioxidant activity after in vitro digestion, probably due to the presence of polyphenols that are released from the matrix during the digestive process.