The pumpkin has great genetic variability and this characteristic directly influences its composition, mainly in the content of carotenoids that are widely found in these fruits. The carotenoids there is an important role in human food because for antioxidant activity and help protect the body against degenerative diseases. The objective was to compare the total carotenoids present in commercial cabotias “Tetsukabuto” (TET) and four experimental hybrids (HC02, HC03, HC05 e HC12) produced in Iporá, GO. The fruits were washed, sanitized and cut manually, separating bark, pulp, seeds and residue, where only the pulp was used later in the analysis. The total carotenoid content was determined through maceration of the sample with acetone and ethanol (1:1) and BHT (20 mg.L-1) solution, the absorbance reading of the obtained extract was performed using a spectrophotometer in 470 nm, value corresponding to the maximum absorbance of the carotenoid lycopene, subsequently quantified in μg of total carotenoids / g of sample (μg.g-1). The means were submitted to ANOVA and Tukey test (p <0.05). There was no significant difference between HC02 (21.71) and HC12 (30.36) pumpkin and, the HC12 and HC05 (39,94) pumpkin. HC03 (52.53) not present a significant difference HC05 and TET (101.27) presented significant difference. Therefore, the experimental genotypes analyzed did not present significant characteristics with the pumpkin TET, but was observed that genotypes HC03 presented carotenoid content close to that obtained in commercial cabotiá TET, showing itself as promising for future studies of genetic improvement, and may become an alternative for the import of Brazilian agricultural seeds. The search for hybrids that have been lost over time is important because it increases the ability of cultivated plants to respond to climate change and other types of biotic and abiotic stresses, that is a serious risk to the sustainability of agriculture.