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SCAVENGING OF REACTIVE OXYGEN SPECIES BY THE CAMPOMANESIA PHAEA PHENOLS

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The consumption of fruits has shown benefits to human health, such as prevention of cardiovascular diseases, showing the importance to study brazilian native fruits. Therefore, the aim of this work was to evaluate the phenolic compounds content and the scavenging of reactive oxygen species (ROS) by cambucí (C. phaea) pulp fruits were collected at Frutas Raras Farm, located at Campina do Monte Alegre-SP. The extraction of total phenolic compounds (TPC) from pulp was prepared with ethanol 80%, (v/v). The TPC were analysed according to Kruawan and Kangsadalampai, (2006). The ROS (ROO•, O2• - and HOCL) were performed according to Chistè et al. (2011) and Melo et al. (2015), using the Spectra-Max M3 microplate (Molecular Devices). All samples were performed in triplicate. The results obtained for TPC and ROO• were 12.11 ±2.01mg GAE g-1 of pulp and 68.94 ±1.78 μmol of TE g-1 of pulp, respectively. The values of TPC and ROO• were higher than those found to bilberry fruits (Vaccinium myrtillus, L.), 5.64 mg GAE g-1. For the O2•- and HOCL, the cambucí presented IC50 of 0.57 ±0.02 mg mL-1 and 0.07 ±0.0 mg mL-1, respectively. The results showed that cambucí is a good source of antioxidant compounds with potential to deactivate ROS, which are related to several diseases.