Nanostructured materials exhibit physicochemical properties that provide the creation of new high-performance materials. The nanofibers have a high surface area in relation to volume and can be used in the protection of unstable compounds and easy degradation. The technique of nanofiber production by electrospinning consists of the application of electrostatic field on the polymer solution. Poly (D,L lactic acid) (PDLLA) has characteristics of biodegradability and certification by the FDA (Food and Drug Administration) for being biocompatible and non-toxic, and can be used in food storage. Phycocyanin is a natural blue biocomposite found in cyanobacteria and red algae, has antioxidant and anti-inflammatory properties enabling its application as a food dye. The aim of this study was to produce PDLLA nanofibers added by phycocyanin by the electrospinning technique for the development of bioactive packages. The polymer solution was prepared using 25% PDLLA and 0.5% phycocyanin and as solvent chloroform:acetone (3:1). The process conditions were feed rate of 150 μL/h, voltage 24.3 kV, capillary diameter 0.45 mm and distance between capillary and collector 150 mm. Scanning electron microscopy, fluorescence and phase contrast evaluated the nanofibers. Thermogravimetric analyzes (TGA) and differential scanning calorimetry (DSC) were performed. Nanofibers of 921 ± 180 nm were obtained and fluorescence and phase contrast microscopy analyzes confirmed the incorporation of phycocyanin to the nanofibers. The polymeric nanofibers provided thermal protection to phycocyanin and from the analyzes performed on the thermograms there was no residual solvent. Thus, the development of phycocyanin-added nanofibers for use as active packaging can extend shelf life by inhibiting product degradation and contributing significantly in the food industry.