The present study developed an improved analytical method for the simultaneous quantification of aflatoxin B1, B2, G1, e G2, deoxynivalenol and zearalenone - the 6 main Aspergillus and Fusarium mycotoxins, in corn. It uses ultra fast chromatography connected to tandem mass spectrometry (UHPLC–MS/MS) and has no clean-up involved. Improvements concern robustness and are especially focused on extraction, chromatographic separation and MS/MS ionization source parameters optimization. Briefly, 5 g of sample were extracted for 3 min with 10 mL acetonitrile:water (84:16, v/v) and the extract was filtered, diluted and submitted to chromatographic analysis (11.5 min). The optimized method offered proper correlation coefficient (r2 > 0.99), precision (RSD always < 12%) and recovery (84-96%). Besides being validated for the 6 referred micotoxins, the method was already extended to other 7 micotoxins (fumonisins B1 and B2, ochratoxin A, nivalenol, diacetoxyscirpenol and T-2 and HT-2 toxins). In addition, preliminary tests have demonstrated potential for expansion to, at least, other 4 toxins (cyclopiazonic acid, fusarenon-X, 3-acetyl-deoxynivalenol and 15-acetyl-deoxinivalenol), thus reaching a total of 17 mycotoxins. This quantitative method has many advantages including simple pretreatment with no clean-up and high sensitivity and throughput. It has proved to have potential to be applied to multi-mycotoxin analysis in complex matrixes. Moreover, the method is able to measure concentrations bellow the maximum permitted levels requested for different legislations worldwide.