HPV PREVALENCE IN CERVICOVAGINAL SAMPLES OF ASYMPTOMATIC WOMEN

Early detection of HPV infection in cervical-vaginal samples in asymptomatic women infers the possibility of preventing cervical cancer. For that reason the purpose of this study was to investigate the presence of genetic material of cervical-vaginal HPV samples in asymptomatic women without histopathological changes. MATERIALS AND METHODS: 50 shaved Samples of cervico-vaginal sexually active cervical-vaginal asymphomatic women between 18 to 30 years, sample DNA has been fully extracted. The detection of viral genetic material was carried out by the qualitative PCR technique with broad spectrum primers, for many viral subtypes, followed by agarose gel electrophoresis. RESULTS: In 53.1% (17/32) of the samples there was the presence of viral DNA by the PCR reaction, in contrast, only 3.1% (1/32) cases were positive in the Pap test. CONCLUSION: The PCR technique is useful in the early diagnosis of HPV infection, even in patients without macroscopic lesions and / or cytological changes. Then it proves that the qualitative PCR test detects the presence of the virus earlier and could be used as a screening method as well as Pap smear, and not be restricted to a confirmatory test as it is currently being used