ACTIVITY RESEARCH ANTIMICROBIAL EXTRACT DICHLOROMETHANE SEED TAMARINDUS INDICA L. FACE TO MEDICAL IMPORTANCE OF STRAINS

The occurrence of infections by multidrug-resistant bacteria (MDR), is an important fact. Due to this, new drugs (plant origin or synthetic) are the aim of several researchs. This study aimed to determine the antimicrobial activity of dicloromentano fraction of seed T. indica, against pathogenic strains. Analyzes were performed at the Laboratory of Natural Products and Biotechnology (LPNBio) located at the State University of Bahia (UESB), campus-Itapetinga BA. Tamarindus´ seeds were dried and crushed, after that dichloromethane was used as solvent by exhaustive extraction. It´s result was filtered and had the filtrates collected periodically. Solvent was removed under reduced pressure on rotavap at 45 ° C temperature, a concentration of 100mg/ml extract was obtained. Minimum Inhibitory Concentration (MIC) were performed by broth microdilution, using different concentrations of the extract for testing (517, 2; 285.6; 142.8; 71.4; 35.7; 17.8; 8.9 and 4.4mg/ml). After 24 hours, all strains were re-cultured to verify that the bacteriostatic activity / bactericide. All tests were made in triplicata. A dichloromethane fraction I. indica showed antimicrobial activity at concentrations from 517.2 to 142.8 mg / mL for all strains: Staphylococcus saprophyticus (ATCC 35552), Staphylococcus aureus (ATCC 43300), Staphylococcus aureus (ATCC 25921); Enterococcus faecalis (ATCC 31299), Pseudomonas aeruginosa (ATCC 27853), Enterococcus faecalis (ATCC 29212). However, strains of S. aureus (ATCC 25921); E. faecalis (ATCC 31299) and E. faecalis (ATCC 27853) MIC found in the last concentration tested up to the final test showed that T. indica dichloromethane fraction had bacteriostatic effect for all bacteria except Enterococcus faecalis that is bactericidal.