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Labile carbon (labile C) is the fraction of soil organic matter that responds most rapidly to changes in management. Its quantification is, therefore, an essential tool for assessing the short-term impacts of land use changes on soil health. Thus, this study evaluated how different land use systems (natural succession - SN, eucalyptus cultivation - EU, and abandoned pasture - PA) and conversion times (9 and 22 years) influenced labile C contents. For this purpose, soil samples were collected at four depths (0.0-10 cm; 10-30 cm; 30-60 cm and 60-100 cm) in a Red-Yellow Latosol in the Doce River Basin (MG), following a systematic design in a 3×2×4 factorial scheme. The labile C contents were determined in the laboratory using the potassium permanganate oxidation method. The results showed that SN had the highest values (p < 0.1) of labile C. Although there was no difference in total content (0-100 cm) between the systems at 9 years, SN already stood out with the highest contents in the surface layer (0-10 cm) in this period, maintaining its superiority at 22 years, when it presented the highest values in the total profile (p < 0.1). Therefore, SN stands out as the most effective land use system for the accumulation of labile C, which indicates a rapid recovery of soil functional health, especially on its surface. It is evident that longer conversion periods (22 years) favor an increase in labile C contents, which are mainly concentrated in the surface layers, decreasing with depth.
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