Microalgae-based carotenoid against melanoma cell lines A375 and B16F10?

This study aimed to investigate the effect of the carotenoid from Scenedesmus obliquus biomass against melanoma cells, utilizing an in vitro model. The impact of the biomass on the viability of A375 and B16F10 melanoma cells was measured, and HPLC-PDA-MS/MS determined the carotenoids absorbed by the cells. Additionally, the impact on reactive oxygen species (ROS) and nitric oxide (NO) concentrations in melanoma cells was evaluated. The results showed that the treatment caused a decrease in A375 and B16F10 cells viability in a dose-dependent manner, with the IC50 value of 70 µg.mL-1 and 39 µg.mL-1, for A375 and B16F10 cells, respectively. During the treatment, the melanoma cells absorbed a total of ten carotenoids. Furthermore, treatment significantly increased the concentration of ROS and NO in A375 and B16F10 cells. Therefore, the carotenoid from Scenedesmus obliquus reduced the viability of A375 and B16F10 melanoma cells, working as cytotoxic and pro-apoptotic agent.