Orange peel is a great source of dietary fibers and polyphenols. Health benefits have been attributed to these bioactive compounds, such as lipid profile improves and hipoglycemic effects. We evaluated the effect of orange peel flour supplementation (OPF) in skinny and induced obese rats. Therefore, 24 Wistar rats were divided in four groups: normal control (NC) fed with Supralab® rodent commercial diet; cafeteria diet (obese control - OC) composed by commercial diet, sweet biscuit, peanut and chocolate (proportion 3:2:2:1); skinny supplemented (SS) fed with NC diet supplemented with 2% OPF; and obese supplemented (OS) fed with OC diet supplemented with 2% of OPF for 9 weeks. Weight gain and diet intake were monitored. Intraperitoneal Glucose Tolerance Test (iGTT) and Intraperitoneal Insulin Tolerance Test (kITT) were realized. After 9 weeks, animals were euthanized and liver and adipose tissues were weighted. Serum glucose, triglycerides, cholesterol, LDL, HDL, AST and ALT enzymes were measured. We done T test among NC and OC groups to confirm the obesity induction, and T test among supplemented groups and controls groups (NC x SS and OC x OS). Diet intake, weight gain and ALT, was not different among groups. Adipose tissues (epidydimal, mesenteric and retroperitoneal) weight, total cholesterol, HDL, LDL, glucose and AST enzyme were higher in OC group compared to NC group. AST level were lower in SS and OS compared to controls. OS group showed lower epididymal adipose tissue, liver weight and LDL levels. iGTT was not different among the groups, but the decay velocity of the glycemic curve in kITT was lower in OC group, showing insulin resistance, and OS group showed improvement of insulin resistance compared OC group. OPF supplementation ameliorates lipid profile and insulin resistance in obese rats and can be used as an ally in the prevention and treatment of obesity.