The objective of this study was to investigate the energy content and nutritional composition of meals consumed at work and associate them with workers’ energy expenditure, level of physical activity, nutritional status, and daily diet quality. The nutritional status was classified according to some anthropometric data and the level of physical activity, according to number of steps. Daily energy expenditure was determined by the basal metabolic rate and physical activity-related energy expenditure. The daily diet quality index was determined by analyzing a five-day food diary and a 24-hour recall of Brazilian workers (n=292). The variables were analyzed by Pearson’s correlation, principal component analysis, and hierarchical clustering analysis. Overweight workers prevailed, and daily energy expenditure differed by gender and work sector (p<0.05). Energy intake at work was appropriate, but the intakes of protein and sodium were high. Considering the workers’ gender and sector, the mean energy intake of 1135 kcal (± 245) did not meet their requirements. The diet quality index of the most workers (87%) was classified as “in need of improvement;” 11.3% had a good-quality diet, and 1.7%, a poor-quality diet. PCA showed that most individuals with higher energy expenditure, energy intake and lipid intake were males. Work sector was positively related to energy expenditure and level of physical activity. Body mass index had a tendency to be associate with gender and type of job and inversely with income. There was no evidence that the food consumed at work influenced the considerable incidence of overweight and obesity in manufacturing-plant workers. The obtained data confirm that weight control, level of physical activity, and food intake are critical factors on workers’ health.