Animal health plays a key role in the food industry and animal disease can lead to loss of profitability and unfeasible activity. The fight against these diseases is done through formulations, such as Oxytetracyclines, which are broad spectrum and low-cost antimicrobials. The objective of the present study was to evaluate the bioavailability of the OXITETRACICLINA formulation after deep intramuscular administration in cattle. Eight healthy crossbred cattle were selected, 4 males and 4 females, aged 12 to 24 months, not medicated in the last 60 days. The dosages were made according to the dosing calculation, the volume of 1 ml for each 10 kg of body weight in a single dose. Blood samples were taken after administration of the treatment at the times: 30 minutes, 1, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 60, 72 hours after treatment. Prior to the application, the blood sample was determined as 0 hour. After collection, the tubes sent to the analytical laboratory. After the analytical evaluation of plasma oxytetracycline concentration, the formulation was found to be bioavailable for 72 hours. Subsequently, the pharmacokinetic parameters were calculated, where: (Cmax) 5.7 μg / mL, (Tmax) of 8.0 hours; (ASC0-t) of 157.8 μg.h / mL, (ASC0-∞) of 164 μg.h / mL; MRT - of 23.2 hours and a (T1 / 2β) of 14.7 hours. Oxitetracycline showed sufficient plasma concentrations to eliminate the bacteria Escherichia coli, Streptococcus agalactiae, Pasteurella multocida, Staphylococcus aureus, Leptospira hardjo, Klebsiella pneumoniae, Bordetella bronchiseptica, and Salmonella Typhimurium, since ASC / MIC ratio values were ≥ 24 for all strains. The use of this drug was efficient for the elimination of disease-causing microorganisms in cattle rearing.