Salmonellosis in humans is associated with consumption of contaminated eggs without heat treated. In Brazil, the prevalent serotypes: Enteritidis, Typhimurium and Heidelberg, are target of sanitary barriers for eggs commercialization. Eggs can be contaminated via transovarian, by the presence of the bacterium in the poultry oviduct and by bacterium penetration through the eggshell. There are few informations concerning the capacity of an egg to get contaminated by Brazilian serovars. This study aimed to evaluate the ability of S. Heidelberg and S. Typhimurium serotypes to penetrate through the eggshell, and their detection in albumin and yolk related to shell thickness. Eggs from SPF (Specific Pathogen Free) laying hens were collected between 45-50 weeks of age. These were artificially contaminated by contact with a damp cotton wool, inoculum (15 x 108 CFU / mL). The tests were made in triplicate of ten eggs, divided to the following groups: negative control, three eggs (without contamination), and the test group, 27 eggs (contaminated). Subsequently they were incubated at 37ºC and their contents were analyzed after four and 24 hours. The evaluation was performed by traditional bacteriology and confirmed by biochemical and serological tests. The treatments were compared with Fisher's exact test using SAS statistical software. For S. Heidelberg in both albumen and yolk at times four and 24 hours the percentage of positives was low, demonstrating that this strain did not survive in hostile conditions of the albumen and had a lower capacity of contamination compared to S. Typhimurium, which had a higher positivity percentage in albumen and yolk. Eggshell thickness had no significant relation with the positivity of the samples. The egg infection model showed that the strains used were able to penetrate the eggshell and multiply in both albumen and yolk in different proportions. In comparison, S. Typhimurium presented higher contamination efficiency.