Brazil is one of the main food producers, and it faces waste problems that occur in all stages of the production chain. The losses occur in the harvest, transport, industrialization and commerce. The organic waste from CEASA/DF has been studied because of its great availability and its potential to production of biogas. This is due to the fact that they are a good source of nutrients for the microorganisms responsible for the biogas production. The objective of this work is to study the biodigestion of these organic residues, using various reaction media conditions, in order to verify the best option for biogas production. The experiments were carried out with temperature control without agitation and the effects of pH and inoculums on the production of the gases of interest was observed. This study revealed the importance of pH adjustment in the reaction medium, since the microorganisms responsible for the biogas production have an optimal range for their development. It was also verified the importance of inoculating the sludge in the middle, since it is responsible for inserting more microorganisms that will be able to produce the gases of interest.