The use of mesenchymal stem cells (MSC) for applications in cell therapy and tissue engineering is favoring a revolution in modern medicine. Since traditional systems are shown to be inefficient to meet the demand, the use of culture spinner flasks systems and microcarriers as an alternative in large-scale cell expansion has been necessary. Although a well-established technique, it still requires adjustments, especially when considering the natural susceptibility of the MSC to the formation of agglomerates, which prevent access to nutrients and hampers cell recovery at the end of culture. It is known that additives such as dextran or its sulfated forms have anticoagulant and antiagglomerating properties, being an alternative to modulate the formation of these agglomerates. This work measured the influence of dextran sulfate (DS) in MSC cultures in spinner flasks. Therefore, were
performed cultures of hMSC-TERT lineage in spinner flask containing Cultispher-S microcarrier concentration of 3 g/L, 50 ml α−MEM supplemented with 15% v/v fetal bovine serum. Results show that 0.01% DS concentration allowed the cultivation of MSC. Although the presence of agglomerates has been observed, those did not reach the diameters observed in the control experiment, allowing a high cell growth by reaching a 13-fold expansion factor.