During the last decade, L-Asparaginase has been used in the treatment of types of leukaemia. Many researchers have studied its molecular features, production, and application, resulting in more than 10000 studies published in the scientific base ISI Web of Science. Currently, this drug is not produced in Brazil, where heavy investments are made to import it. Facing this context, a group of Brazilian researchers proposed to develop the production steps of this biopharmaceutical. This work aims to contribute to the group by analysing the huge amount of information published in ISI through a bibliometric study and provide insights that can guide the group studies. Applying statistical and qualitative tools, it was possible to identify the main publisher journals and countries, the evolution of publications and themes, and the hot topics. This overview about L-asparaginase production can guide researchers to focus on areas that need more development.