The phosphorus absorption is essential to plants and encourages the study of alternative mechanisms for providing its soluble form. The use of chemical fertilizers for increasing the cultures productivity on the phosphorus deficiency soil could be replaced by biological processes in the system soil/plant realized by phosphorus solubilizing microorganisms (PSM). Therefore, the objective of this study was to evaluate the phosphorus biossolubilization process from phosphate rock concentrate by Trichoderma harzianum in air-lift bioreactor employing reactive medium recycle. During 6 days was evaluated the phosphorus biossolubilization process by Trichoderma harzianum in air-lift reactor employing ascending air 0.5 vvm and reaction medium recycle containing phosphate rock concentrated (5 g/L). Were, daily, were evaluated the soluble phosphorus concentration and the metabolites concentration using 400 ml/min of reaction medium recycle flow. By employing Trichoderma harzianum in air-lift reactor with reaction medium recycle obtained 216.10 ppm of phosphorus soluble on the third day of process when it occurs the maximum acid organic concentration (lactic and acetic) equal to 8.2 μmol/mL.