Microbial enzymes can act effectively in different processes, such as, the extraction of essential (vegetal) oil. In this work, a crude multi-enzymatic extract - obtained from Aspergillus niger previously cultivated on forage palm - was evaluated for the rupture of glandular trichomes of fresh leaves of Mentha arvensis in order to improve the extraction of its essential oil. By scanning electron micrography it was possible to observe that the structure of trichomes was better preserved with just water (500 ml) as a pre-treatment, regardless the temperature and time. However, with the enzymatic pre-treatment the extraction was improved, for example, 194% more essential oil (0.2831 g from 30 g of fresh leaves) at 36°C and 126 min and with 55 ml (455 ml of water) of the crude multi-enzymatic extract.