In the present study, purification of a red pigment produced by Talaromyces minioluteus in aqueous two phase sytems (ATPS) of PEG/phosphate were investigated. The influence of polymer molecular weight (400, 1000 and 6000), pH (6,2 and 8,0), tie-line length (TLL) (30 and 43) and tie-line ratio (rTLL) (0,75, 1 and 1,25) were studied. PEG molecular weight and rTLL were the variables that most influenced the extractions. The highest PF (purification factor) and CF (concentration factor) were obtained using rTLL 0,75. The highest PF (ranging from 1,2 to 1,4) were observed for systems using PEG 1000 and PEG 6000, at pH 6,2. Only systems using PEG 6000 with TLL 30 and rTLL 0,75 were able to concentrate the pigment, 1,4 times at pH 6,2 and 1,9 times at pH 8,0. Pigment recoveries in the top phase were high in all systems evaluated, ranging from 82 to 100%. This study showed that ATPS, under certain conditions, provided pigment purification and concentration, and it is a promising operation for the downstream process of this bioproduct.