Dextransucrase is an industrial enzyme used to the production of dextran and oligosaccharides. This enzyme presents many difficulties to immobilize, due to dextran masking its reactive groups. To facilitate immobilization dextransucrase was co-immobilized with dextranase in the epoxy-agarose carrier. The synthesis of oligosaccharides was evaluated at different temperatures (4 °C and 25 °C). The results showed that the better conditions of synthesis to the immobilized enzymes was at 25 °C, resulting in low levels of dextran and oligosaccharides with a degree of polymerization up to 10.