Proteases are considered enzymes of great importance given their wide application in several industrial departments, with the solid state fermentation being highlighted in the production of the enzyme as well as the use of agroindustrial byproducts. The literature describes Aspergillus oryzae as one of the largest producers of this enzyme class for its ability to produce acidic, neutral and basic proteases, mainly in the solid state. Therefore, this work has the objective the kinetics study of protease production from the use of the mesocarp, endocarp and babassu cake, using the filamentous fungus Aspergillus oryzae CCBP 001. A study was carried out using an experimental design with binary and ternary mixtures. The substrates evaluated presented proteolytic activity, with emphasis on the babassu cake, producing higher values than those produced by the mesocarp and endocarp of babassu substrates.