In this work, chitosan-based 3D support (CHI) was produced, characterized morphologically and the influence of this material as a support for physical adsorption immobilization of Burkholderia cepacia lipase was evaluated. The 3D structure carrier was lyophilized, neutralized and cross-linked, with diameters of 90 mm and height of 40 mm. The CHI was characterized by analysis by Fourier transform infrared spectroscopy, thermogravimetric analysis and scanning electron microscopy. The immobilization yield of 34% and enzymatic specific activity of 188 U/g were preliminarily obtained, using the loading of 0.025 g lipase to 0.1 g support. SEM images showed a porous structure which is desirable for immobilization by adsorption. A characteristic FTIR band was obtained for the amides demonstrating the efficiency of immobilization of lipase from B. cepacia.