Crude extracts obtained from Urena lobata L. were submitted to anti-leukemia assays. Among them, dichloromethane extract from the aerial parts was selected to start our chemical approach. Based on this, the study is being performed using two rapid dereplication strategies: Target Analysis (Bruker Daltonics®) MS data analysis combined with MS/MS data obtained from GNPS platform. Preliminary data showed the presence of different classes of Natural Products in the cytotoxic extracts. The combination of hyphenated techniques may increase the efficiency and speed of analysis, being useful tools to access unrevealed natural products (NPs), which include molecular dereplication ^1,2. In this sense, our goal is the employing of integrated analytical techniques to accelerate the discovery of metabolites (unknown or not) from Urena lobata L., guided by anti-leukemia assays. For this, different human leukemia cells were employed in an initial screening: Raji (Lymphocyte B), CCRF-CEM (Acute Lymphoblastic Leukemia) and Kasumi-1 (Acute Myeloid Leukemia). The cytotoxic assays (during 24 hours at 100 μg/mL) allowed us to select the dichloromethane extract from the aerial parts of U. lobata L. (ULD). After a chromatographic column (on silica flash), ten subfractions from ULD (2,0 g) were obtained; all of them were submitted to the cytotoxic evaluation again. Most of them practically inhibited at least 85% of the leukemia cells proliferation. Our UHPLC-MS analyzes revealed the presence of more than 100 ions present in the subfractions. Through the integrated tools we were able to find some compounds related and also unrelated in the literature for the species, for example, urenalobaside A, glycosilated urenoside and hydroquinidine which reveals, therefore, good perspectives for discovering anti-leukemia and/or unrelated NPs from U. lobata L.

¹ Carvalho, A.; Rodrigues, L.; Ribeiro, A.; Silva, M.; Medeiros, L.; Veiga, T. Integrated Analytical Tools for Accessing Acridones and Unrelated Phenylacrylamides from Swinglea glutinosa. Molecules 2020, 25, 153 ² Van Herwerden, Eric F.; Süssmuth, Roderich D. Sources for Leads: Natural products and libraries. In: New Approaches to Drug Discovery. Springer International Publishing, 2015. p. 91-123.