Bees are pollinating agents that find in the nectar and pollen as their source of food and energy. They belong to the order Hymenoptera, of extreme importance for the conservation of plant and animal species, since they house the largest number of pollinators. Pollination is one of the most important mechanisms for maintaining biodiversity, and in this scenario, according to the United Nations Food and Agriculture Organization (FAO), it is estimated that of the 100 species of crops responsible for providing 90% of the food in the world, 71 are pollinated by bees. In addition, bees are responsible for the production of honey and other supplies, such as wax and propolis, which contribute to human well-being. Since the 80's and 90's there has been a gradual reduction in the number of bees, but in a small magnitude. Currently, these insects are at high risk of extinction, and it is believed that a combination of several factors causes this population decline, such as deforestation, climatic variations, pathogens (viruses, bacteria, mites) and use of pesticides. Contamination of bees by pesticides can result from the negligent use of farmers, for example, by striking them for imagining that they harm the crop. Pesticide residues can be absorbed by bees during the collection of nectar and/or pollen and water. This project aims to seek the correlation between bee mortality and the use of pesticides, through modern chromatography techniques coupled with mass spectrometry, which will support the attainment of metabolic profiles subsidizing the search for pesticide residues in these bees. This because it is extremely important to assess the reach of pesticides in the environment and understand what impact they cause on bees and other pollinators. To achieve this proposed objective, several methods of extraction and analysis were selected in order to obtain the best methodology.