NMR-based metabolomics in biophotonics

Low-Level Laser Therapy (LLLT) is widely used clinically for tissue repair, inflammation reduction, pain relief, and treatment of cosmetic dysfunctions. LLLT acts on the absorption of photons by chromophores at the cellular, molecular and tissue levels, resulting in increased cellular activities, cytokine production, growth factors, proliferation, differentiation, and cell migration. However, little is known about the metabolism linked to photobiomodulation. Our work focuses on map the cellular and metabolic profile of skin cells (FGH-fibroblast and MV3-Invasive metastatic melanoma) after irradiation with red laser, 660 nm, 100 mW, with 35 and 75 J/cm2, same protocol for pression ulcer. We did exploratory NMR-based metabolomics to map the intra and extracellular metabolic profile. Also, we evaluate the cells viability by MTT assay, the growth behavior, apoptosis and necrosis by annexin/PI, and cell migration by wound healing. Our results showed the red LLLT increased up to 16% in cell viability in MV3 cells after treatment, as well as a significant change in the energy consumption of the cell, since there are changes in glucose and lactate levels found in cell extracts. Other results will be shown in the poster session.