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This study aimed to evaluate the influence of different in vitro culture media, MS, Y3 and MS medium with activated charcoal on the germination and development of Acrocomia aculeata embryos. The study aimed to identify the best solution for in vitro culture, to help strengthen the macaúba production chain. An experiment was carried out in the laboratory using three types of culture media and multiple replications. 60 tubes were used for each medium, with one macaúba embryo per tube. The embryos were kept under controlled conditions and in the absence of light, until their germination, after germination, the seedlings were kept in growth rooms with light availability. Development was monitored weekly, and plant growth was measured at the end. After one month of the experiment being established, it was possible to analyze the beginning of germination, both in the MS medium and in the Y3 medium there were approximately 27% of germinated embryos, while the MS medium with activated charcoal obtained 30% germination rate. In the second month, an increase in germination was observed, the MS treatment obtained 30% at the end of the month, 35% DM with activated carbon, and finally Y3 presented the highest rate equivalent to 40%. The development of the plant was observed from the third month onwards, and the treatment that led to the best results was the DM with activated carbon, while the Y3 medium was responsible for the etiolation of the plants
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