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Karina Mayumi Tani Bezerra de Melo replied to the topic "A question about the results"

Publication: INTERFERENCE RNA FOR PERSONALIZED CANCER THERAPY: WHERE ARE WE NOW?

First, I'd like to say that your presentation was very clear and informative. I just have one question: are these RNA interference therapies, in which a sample is collected from patients and the epigenetic study is performed, already being conducted? If so, can you tell me if there are any specific studies on breast cancer? I'm particularly interested in that.

Question

Karina Mayumi Tani Bezerra de Melo replied to the topic "Question"

Publication: INTERFERENCE RNA FOR PERSONALIZED CANCER THERAPY: WHERE ARE WE NOW?

Very interesting presentation! How close are we to implementing RNAi as a routine treatment option for cancer patients?

Question

Celina Yung-Ai Lin Lee replied to the topic "Methodology "

Publication: Combined brachydins, A, B, and C, induced cytotoxicity by the activation of the intrinsic mitochondrial apoptosis pathway

Hello, Celina, good evening! Congratulations on your presentation and the excellent work! I would like to know, in order to verify this selective effect on cancer cells, do you intend to observe the effects of brachydins on non-tumoral breast cells, such as MCF-10A? Do you plan to observe these effects in another breast cancer cell line? And do you intend to analyze the protein levels of the genes analyzed by RT-qPCR? Thank you!

Question

Mariana Teixeira de Freitas replied to the topic "qRT-PCR"

Publication: The Antagonistic Role of DHMEQ, an NF-κB Inhibitor, in Breast Cancer Progression

Very interesting research! I wonder by which approach (Sybr or Taqman) you'll examine the NF-kB signaling. Also, which targets do you intend to test? Thanks!

Question

Mariana Teixeira de Freitas replied to the topic "methodology"

Publication: The Antagonistic Role of DHMEQ, an NF-κB Inhibitor, in Breast Cancer Progression

Are you considering analyzing members of the NF-κB related pathway, affected by DHMEQ?

Question

Isabel Cristina Pimentel dos Santos replied to the topic "Other types on cancer "

Publication: Characterization of the Response to Treatment with 5-Fluorouracil Combined with the APE1 Inhibitor, APX2009, in Triple-Negative Breast Cancer Cells

Congratulations on your work, Isabel. Selectivity is one of the key factors in treatment. Do you consider that the positive outcome of this combination may be related to the type of cancer addressed in your study?

Question

Ana Caroline da Silva Ferreira replied to the topic "Methodology "

Publication: Effects of Photobiomodulation by Infrared Radiation on Cancer Hallmarks in Cellular Regulatory Pathways - Review

Hello, Ana Caroline, good evening! Congratulations on your presentation! I would like to know if, depending on the physical irradiation parameters used, these results could be different? Also, do you plan to study which possible pathways are related to the processes mentioned in the results?

Question

Ana Caroline da Silva Ferreira replied to the topic "New methodologies"

Publication: Effects of Photobiomodulation by Infrared Radiation on Cancer Hallmarks in Cellular Regulatory Pathways - Review

Good morning, great work, Ana Caroline. Very informative. My question is, do you intend to test this observed data by conducting experiments with a specific type of cancer?

1 person favorite the paper "Characterization of the Response to Treatment with 5-Fluorouracil Combined with the APE1 Inhibitor, APX2009, in Triple-Negative Breast Cancer Cells "

Suggestion

Anna Gabriele Prado dos Santos replied to the topic "MMP abundance"

Publication: CIRSIMARIN MODULATES A549 LUNG CANCER CELL MIGRATION IN 2D AND 3D CULTURES VIA TRANSCRIPTIONAL REGULATION OF METALLOPROTEINASES

Congrats for the amazing presentation and interesting work Anna! I have a suggestion to improve the robustness of the work. You have seen in your study differences in gene expression of MMP2, MMP9 and MMP11, but it would be interesting to investigate/prove that these alterations in MMP's gene expression is accompained but differences in MMP's abundance. I would like to suggest a highly reproducible and cheap experiment that is gelatin zymography.

Question

Karina de Menezes Leitão replied to the topic "Next steps"

Publication: The Role of the FBXW2 Enzyme as a Potential Modulator of NF-κB Activity and Its Influence on the HER2+ to Triple-Negative Subtype Transition in Breast Cancer

Your work is very interesting! What are the next steps you have planned?

Question

Tainá Miotto de Souza replied to the topic "Questions"

Publication: DNA methylation profile in pediatric patients with germ cell tumors

Thanks for your amazing talk and results! I have some questions, as follows: 1) Did you identify subtype-specific methylation patterns (e.g., yolk sac vs dysgerminoma vs immature teratoma), and if so, do these overlap with known methylation “imprints” in primordial germ cell development or pluripotency pathways? 2) Since resistance to cisplatin is a major clinical issue, have you explored whether methylation changes in DNA repair genes (e.g., MGMT, MLH1, or other repair-associated promoters) correlate with high-risk or resistant cases in your cohort? 3) Considering the reliance on tumor markers and histopathology in GCT diagnosis, do you envision that DNA methylation profiling could be translated into a minimally invasive diagnostic approach (for instance, via circulating tumor DNA methylation in plasma) and how would you address challenges of sensitivity and specificity in children? Thanks and congrats again!

Question

Tainá Miotto de Souza replied to the topic "Implications of DNA methylation in tumor biology"

Publication: DNA methylation profile in pediatric patients with germ cell tumors

Congratulations on your work, it's so complex and well-developed! I work with gliomas, which are brain tumors, and there's much discussion about methylation changes. Some gliomas even present a hypermethylator profile, so this is a very promising study. I just have two questions regarding your work: 1. Your methylation data from 13 pediatric GCT samples are very interesting. Considering the small sample size and tumor heterogeneity, have you thought about ways to validate whether these patterns are consistent across a larger patient population or other tumor subtypes? 2. Have you considered complementing this with RNA-seq or proteomics to explore how these methylation changes might influence gene expression or tumor behavior? Again, congrats for your project!

Question

Anna Gabriele Prado dos Santos replied to the topic "Questions"

Publication: CIRSIMARIN MODULATES A549 LUNG CANCER CELL MIGRATION IN 2D AND 3D CULTURES VIA TRANSCRIPTIONAL REGULATION OF METALLOPROTEINASES

Thanks for your amazing talk and results! I have some questions, as follows: 1) You demonstrated the downregulation of MMP2/9/11 transcripts after cirsimarin exposure, so I wonder if these transcript changes translate into reduced proteolytic activity in the extracellular milieu, for instance, and have you measured their active vs. pro-forms (e.g., by zymography or activity assays)? 2) There is a notable difference in effective concentrations between your 2D (1–40 μM) and 3D (160 μM) assays. How do you interpret this in terms of compound diffusion, binding to ECM components, local aggregation/solubility, or compound stability within spheroids? 3) Which upstream signalling pathways do you hypothesize link cirsimarin to MMP transcriptional repression — for example, FAK/ERK, NF-κB, or TGF-β/EMT regulators?

Question

Isabel Cristina Pimentel dos Santos replied to the topic "Differential Sensitivity of HCC-1937 and MDA-MB-231 Cells"

Publication: Characterization of the Response to Treatment with 5-Fluorouracil Combined with the APE1 Inhibitor, APX2009, in Triple-Negative Breast Cancer Cells

Very good work and presentation, Isabel, congratulations!. My question is about which molecular mechanisms could explain the greater sensitivity of the HCC-1937 cell line compared to MDA-MB-231 when treated with the combination of 5-Fu and APX2009?

1 person favorite the paper "CIRSIMARIN MODULATES A549 LUNG CANCER CELL MIGRATION IN 2D AND 3D CULTURES VIA TRANSCRIPTIONAL REGULATION OF METALLOPROTEINASES "

1 person favorite the paper "DNA methylation profile in pediatric patients with germ cell tumors"

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